Oomycetes Soybean EST Database

Oomycetes, Soybean ESTs

PhESTDB is an integrated resource hosting combined EST data from Oomycetes such as P.sojae, P.infestans and H.parasitica. Apart from that we have separated Soybean ESTs from compatible P.sojae infection libraries. Public soybean unigenes and publicly available soybean infection libraries are also now part of this database.

Currently in this database we have placed 10 libraries from P.sojae(sHA,sHB,sMA,sML,sMY,sMC,sZG,sZO,sZS,iMY) having 33350 raw sequences. Additionally, there are 99,320 EST sequences from P.infestans from NCBI. For P.infestans sequences cleaning information is not available. These sequences are clustered and assembled and data analysis was performed by us. We have separated Soybean ESTs from P.sojae libraries using insillico methods. The soybean libraries are named as gHA and gHB based on their origin from sHA or sHB.

All publicly avaialble soybean unigenes(37,465) are added to this database. For public soybean sequences we did not perform any cleaning or assembly. The unigenes were annotated prior to storing. Recently, we added 37492 ESTs from Hyaloperonospora parasitica. All the ESTs are clustered into unigenes and annotated using blastx to nr database, interproscan, TMHMM and SignalP. Annotations are updated every 3 months.