EMBOSS

BLAST

PAIRWISE SEQUENCE ALIGNMENT

ANALYZE SEQUENCE

BUILD WEIGHT MATRIX

CALCULATE DIPEPTIDE FREQUENCY

SUB SEQUENCE

Select Blast Program

Select Database

Evalue(E): Cutoff Value(S):

Substitution matrix BLOSUM62 BLOSUM40 BLOSUM50 BLOSUM60 BLOSUM70 BLOSUM80 BLOSUM90 BLOSUM100 PAM30 PAM70 Maximum Alignments(B): 10 20 50 100 250